Complete nucleotide sequence of the simian rotavirus SA11 VP4 gene
نویسندگان
چکیده
منابع مشابه
Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence.
DNA sequences complementary to the double-stranded RNA coding for the neutralization antigen (glycoprotein VP7) of simian rotavirus SA11 have been cloned. The VP7 gene consists of 1,062 nucleotides, containing an uninterrupted coding sequence of 978 nucleotides which specifies a glycoprotein of 326 amino acids. The significance of a second possible initiation site 30 nucleotides downstream from...
متن کاملPreferential Assortment of Rearranged NSP3 Gene in the Genetic Background of Simian Rotavirus SA11
Selective assortment of rotavirus NSP3 gene in the genetic background of simian rotavirus strain SA11 was analyzed by using single-NSP3 gene-substitution reassortants having NSP3 gene from human rotavirus strain KU (KU-R) or porcine rotavirus strain OSU (OSU-R) in the SA11 background, and an SA11-derived variant Ga613 having a rearranged SA11NSP3 gene which is 937 nucleotides longer than the or...
متن کاملCloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
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Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...
متن کاملEffect of actinomycin D on simian rotavirus (SA11) replication in cell culture.
Rotaviruses are the major cause of viral diarrhea in humans and animals. Actinomycin D (Act D) is an antibiotic that intercalates DNA and therefore inhibits DNA-dependent transcription. The current study was carried out to assess the influence of Act D on the replication of simian rotavirus (SA11) in cell culture. Virus-infected MA-104 cell cultures were studied in the presence of Act D at conc...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.5.2122